Office:
327E LappinBIOL 304: Genetics
Fall 2005: T/Th 10:20 Lappin 307
Instructor: Dr. David Peyton
Office:
327E Lappin
Office hours: MTW 8-10am in 327E or 322 (lab)
Phone: 783-2947
Email: d.peyton@moreheadstate.edu
Required Textbook: iGenetics by Peter J. Russell, (2002)
Course Objectives:
The primary course objective for BIOL 304 is to understand the genetic basis of life, in this case summarized under Ten Principle Topics. Virtually every biological activity, normal and pathological, has a genetic origin. This is true for inherited disorders as well as disorders that arise during development or during the lifetime of an individual. By understanding the molecular basis, the biological systems of the body can be seen as an organized collection of chemical events that are fine tuned but also susceptible to malfunction and mutation. During the semester we will attempt to master these concepts as they occur under the Ten Principle Topics:
The Ten Principle Topics in order of their presentation:
1. Basic genetic ideas, starting with the discovery of a genetic “unit” and the structure of DNA.
2. Basic (normal) genetic function: the central dogma in detail, from replication to transcription to translation.
3. Regulation of transcription as pertains to gene control under different circumstances.
4. Cell cycle control and a detailed look at the cyclins and cyclin dependent kinases.
5. Aberrant cell cycle leading to cancer.
6. Experimental techniques that are used to study genetics and other basic biology questions.
7. Application of these techniques in therapy.
8. Human genome issues (technology and ethical issues).
9. Genetic basis of inherited diseases and their transmission.
10. Population genetics and speciation.
Course Policies:
Success in this class will depend on understanding the material, not just memorizing it. If you are unsure about lecture or reading material, make a point to ask a question during or after class. Lab sessions will also provide opportunities to review class material, and I am available in my office for discussion of specific problems or class topics.
Attendance is required and you are expected to read assigned material before class when appropriate. Quizzes given during class time will be a large percentage of your grade. There is no quiz makeup but a maximum of two quizzes can be missed without penalty (if you are present for all quizzes then the lowest two quiz grades will be dropped). Quizzes are unannounced but there will be one per week covering material from previous lectures, this includes exam weeks!
Lab exercises will be another component of your grade and will be handed out or assigned during the lab. If you have an excused absence during a lab session, you are still responsible for the assignment given during that session. Lab assignments will not be graded for students with an unexcused absence for that period.
There are four exams (three will be taken during lab sessions, the fourth is the final). Makeup exams (for excused absences only) must be taken the same week as the missed exam.
Please practice common courtesy in the classroom. Students are encouraged to speak up and ask questions during class, but please raise your hand so that we are not competing. Please refrain from conversation with your neighbors as this is distracting to those around you who might be more interested in the lecture. Cell phones are absolutely forbidden in class. Turn them off! Incoming calls will cost the receiver 10 points per ring. Class will start on time according to the clock in the classroom, and quizzes will begin promptly at 10:20. Latecomers will not be allowed to make-up the quiz, and will irritate the professor! Taking notes on a laptop during class is not permitted in this course.
Exams 100pts x 4 = 400
Quizzes 10pts x 14 = 140
Total points possible 600
Diversity issues:
This course addresses the diversity of organisms and humans in the context of genetic makeup and inheritance.
Disability statement:
Any student who feels that he/she needs an accommodation for any kind of disability should make an appointment with me as soon as possible in order to discuss this issue.
Kentucky's New Teacher's Standards:
Genetics 304 will offer a constructive, flexible learning environment (NTS II) in which students will gain content knowledge in the area of genetics (NTS VIII). Students will be encouraged to participate as active learners through question and answer sessions at the beginning of each class meeting and through discussions in the lab component of this course. Group activities and a written exam component will be used to foster effective communication with peers and colleagues (NTS III). Students will be encouraged to use technology in the form of computer-generated course materials (NTS IX) supplied with their text. The effectiveness of instruction (NTS V) and learning (NTS IV) will be assessed through student exams, quizzes, homework assignments, and student interactions. Students should approach this course with the concept of the student as an architect, building on previous experience to shape their own understanding of the core topics.
Lecture Schedule for Fall 2005:
WEEK DATE CHAPTER LECTURE TOPIC
EXAM 1 Material:
1. Aug 23-25 Chapter 1 Assessment exam, Mendel’s principles, central dogma, mitosis/meiosis
2. Aug 30-Sep 1 Chapters 2,3 DNA chemistry, bases, history and discovery, replication
3. Sep 6-8 Chapter 5 Transcription, gene structure, RNA processing
4. Sep 13-15 Chapters 6,16 Translation
EXAM 2 Material:
5. Sep 20-22 Chapter 16 Lac operon, chromosomal regulation
6. Sep 27-29 Chapters 17,18 Transcription factors, cell cycle basics
7. Oct 4-6 Chapter 18 Cell cycle regulation, cancer genetics
8. Oct 11-13 Chapter 19 Types of cancer, DNA repair mechanisms
EXAM 3 Material:
9. Oct 18-20 Chapters 7-9 Experimental genetics, northern, southern, and western blots
10. Oct 25-27 Chapters 7-9 Experimental assays continued
11. Nov 1-3 Chapters 7-9 DNA sequencing, PCR, forensics, Human genome project
12. Nov 8-10 Chapters 7-9 Transgenic mice and knockout mice
EXAM 4 Material:
13. Nov 15-17 Chapters 21,11,12 Genetic diseases, chromosomal abnormalities, autosomal inheritance
14. Nov 22 Chapters 21,11,12 Sex-linked inheritance, pedigrees, Thanksgiving Break
15. Nov 29-Dec 1 Chapters 21,11,12 Hardy-Weinberg, population genetics
16. Dec 6-8 Chapter 22 Bottleneck, genetic drift, speciation, review
***** Final Exam: Tuesday Lab (Section 001): Monday, Dec. 12th @ 12:45pm-2:45pm
Wednesday Lab (Section 002): Tuesday, Dec. 13th @ 12:45pm-2:45pm
Lab Topics for Fall 2005:
WEEK DATE TOPIC
1. Aug 23-24 Introduction, pipetting, DNA structure and strand arrangement, prepare media and LB plates
2. Aug 30-31 Mini-prep DNA isolation, restriction enzyme digestion
3. Sep 6-7 Gel electrophoresis, restriction mapping of unknown DNA
4. Sep 13-14 Fish DNA amplification, Exam 1 review
5. Sep 20-21 Exam 1 given during lab meeting
6. Sep 27-28 Gel electrophoresis and ligations of PCR clones, transform competent DH5alpha cells
7. Oct 4-5 Mini-preps, set up restriction digests
8. Oct 11-12 Gel electrophoresis and analysis, cell cycle/general review for Exam 2
9. Oct 18-19 Exam 2 given during lab meeting
10. Oct 25-26 Roadkill DNA preparation, PCR amplification of actin sequences
11. Nov 1-2 Bioinformatics exercise
12. Nov 8-9 Electrophoresis of mammal PCR samples, distance calculation, Exam 3 review
13. Nov 15-16 Exam 3 given during lab meeting
14. Nov 22-23 Thanksgiving Break
15. Nov 29-30 Human DNA isolation, TPA allele analysis for heterozygosity/homozygosity
16. Dec 6-7 Electrophoresis of TPA samples, Hardy-Weinberg exercise, Final Exam review
Exams 100pts x 4 = 400
Quizzes 10pts x 14 = 140
Total points possible 600
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Exam 1 |
Lab 5 |
Quiz 1 |
Quiz 9 |
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Exam 2 |
Lab 6 |
Quiz 2 |
Quiz 10 |
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Exam 3 |
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Quiz 3 |
Quiz 11 |
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Exam 4 |
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Quiz 4 |
Quiz 12 |
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Lab 1 |
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Quiz 5 |
Quiz 13 |
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Lab 2 |
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Quiz 6 |
Quiz 14 |
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Lab 3 |
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Quiz 7 |
Quiz 15 |
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Lab 4 |
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Quiz 8 |
Quiz 16 |