Research

 

Laboratory Protocols

Sunfish Identification Using PCR

GenBank Files:

Esox masquinongy growth hormone gene, exons 2 through 6

Esox lucius growth hormone gene, intron D

Esox reichertii growth hormone gene, intron D

Esox niger growth hormone gene, intron D

Esox americanus growth hormone gene, intron D

Publications:

            Manuscript submitted to Journal of Kentucky Academy of Science: Peyton, D.K. “The Beta-actin Gene in Esocids and Independent Evolution of the Actin Gene Family Members.”

            Manuscript submitted to Transactions of the American Fisheries Society:  Kara R. Barnett, Kristopher Fultz, Brent Kidd, Rebecca Yates, David K. Peyton.  “A Rapid, Single Gene Test to Identify Sunfish and Their Hybrids.”

            Manuscript submitted and under review to Copeia journal:  Barnett, K. R. and Peyton, D.K.  “A Minisatellite in the Growth Hormone Gene of Esocidae is Derived from a Single Copy Element in the Salmonid Genome.”

            “A PCR-Based F1 Hybrid Screen Using the Beta-actin Genes From Lepomis cyanellus and Lepomis macrochirus.” David K. Peyton.  Journal of the Kentucky Academy of Science. (2004) 65,2.

            “The Alpha-fetoprotein Promoter is the Target of Afr1-Mediated Postnatal Repression.”  David K. Peyton, Mei-Chuan Huang, Meredith A. Giglia, Nedda K. Hughes, and Brett T. Spear.  Genomics 63, 173-180 (2000).

            “Position-dependent activity of alpha-fetoprotein enhancer element III in the adult liver is due to negative regulation.”  David K. Peyton, Tennore Ramesh, and Brett T. Spear.  Proceedings of the National Academy of Sciences 97, 10890-10894 (2000).

Recent presentations:

            Barnett, K. R. and Peyton, D.K. “A Minisatellite in the Growth Hormone Gene of Esocidae is Derived from a Single Copy Element in the Salmonid Genome” American Society of Ichthyologists and Herpetologists Annual Meeting, July 6-10th, 2005, Tampa, Florida.

            Barnett, K. and Peyton, D. “Molecular Phylogeny of Esocids and the Discovery of a Minisatellite in the Growth Hormone Gene of Esox masquinongy.”  Honor’s Day, Morehead State University, April 16th, 2005.

            Barnett, K. and Peyton, D. “Molecular Phylogeny of Esocids and the Discovery of a Minisatellite in the Growth Hormone Gene of Esox masquinongy.”  Kentucky Academy of Sciences (KAS), November 2004

            Kidd, B., Fultz, K., Yates, B. and Peyton, D. “Using Nuclear Gene Polymorphisms to Identify Lepomis Species and Lepomis Hybrids.”  KAS, November 2004.

            Morgan, V., Peyton, D.K., and Wymer, C.L. “Vegetative Propagation of Japanese Knotweed.”  Oral presentation by Virginia Morgan at the KAS, November 2003.

            Peyton, D.K., Fountain, D., Dean, J., Stauble, J.S. and Spear, B.T. “The DNA-Binding Domain of HNF-3beta Represses the AFP Promoter.” Undergraduate poster presentation by Scott Stauble at the Posters at the Capital meeting, Frankfort, KY, February 2003.

Scholarly activity:

            Submission of R15 AREA grant to the National Institutes of Health, September 2005:  “The Influence of Minisatellite Expansion at a Splice Regulatory Site.”

            IRAPP Grant for January - December 2005:  “Polymorphisms in the Growth Hormone Gene of the Pike Family (Esocidae)”  Funded for $5,000.

            Continuation of INBRE Grant for July 2005-June 2006:  “Molecular Mechanisms of Estrogen-Regulated Bone Resorption.”  Co-investigator with Darrin DeMoss.

            IRAPP Grant for January – December 2004:  “Characterization of the Growth Hormone Gene in Muskellunge, Esox masquinongy.”  Funded for $4,360.

            INBRE Grant for September 2004-July 2005:  “Molecular Mechanisms of Estrogen-Regulated Bone Resorption.”  Funded for $153,468.  Co-investigator with Darrin DeMoss.

            IRAPP Grant for January – December 2003:  “The use of DNA fingerprinting to determine the relatedness of Japanese Knotweed (Fallopia japonica) populations in Eastern Kentucky.” Co-investigator with Carol Wymer.  Funded for $4,958.

            KBRIN Faculty Collaborative Research grant for April – August 2003:  “Critical Binding Domain of Hepatocyte Nuclear Factors.” Funded for $11,000.

            KBRIN Grant for September 2003-July 2004:  “Molecular Mechanisms of Estrogen-Regulated Bone Resorption.”  Funded for $81,053.  Co-investigator with Darrin DeMoss.

            National Science Foundation EPSCoR grant for 2002-2003:  “Molecular Aspects of Adventitious Root Formation in Fallopia japonica” funded in the amount of $20,369.  Co-investigator with Dr. Carol Wymer.

            KBRIN Faculty Collaborative Research grant for 2002:  “Regulation of the AFP promoter” funded in the amount of $10,000.

            University-funded grant for 2002:  “Determination of the Critical Region of the Hepatocyte Nuclear Factor 3 Protein” funded in the amount of $4782.